作者：耿琦1, 余洋1, 刘绒梅1, 田圆圆1, 李玺洋1, 梁鹏宽1, 石海春2, 柯永培2, 孙群1
作者单位：1. 四川大学生命科学学院 生物资源与生态环境教育部重点实验室, 四川 成都 610064;
2. 四川农业大学农学院, 四川 成都 611130
关键词：淡紫灰链霉菌s3-5;田间试验;稳定剂;紫外保护剂
摘要：
尾孢菌（cercospora zeae-maydis）是引起玉米灰斑病的病原真菌，淡紫灰链霉菌（streptomyces lavendulae ）s3-5为课题组前期研究筛选出的尾孢菌拮抗菌株。为探究s3-5在田间对玉米灰斑病的防治效果，并研制有效防控玉米灰斑病的生防制剂，该研究进行田间试验，并对s3-5稳定剂及紫外保护剂进行筛选。结果表明，初步应用s3-5孢子悬液于田间栽培品种正红311上，发现其对玉米灰斑病具有一定的防治效果，喷施两次的效果更显著（p0.05）；最佳稳定剂为2.5%甲基纤维素，最佳紫外保护剂为0.3%异维c钠。可为s3-5制剂的后续研究奠定基础。
effect exploring of anti-gray leaf spot on maize in the field and screening of adjuvants for the biocontrol agent of streptomyces lavendulae s3-5
geng qi1, yu yang1, liu rongmei1, tian yuanyuan1, li xiyang1, liang pengkuan1, shi haichun2, ke yongpei2, sun qun1
1. key laboratory of bio-resource and eco-environment of ministry of education, college of life sciences, sichuan university, chengdu 610064, china;
2. agronomy college, sichuan agricultural university, chengdu 611130, china
abstract: cercospora zeae-maydis is the cause agent of gray leaf spot disease (gls) in maize, and streptomyces lavendulae s3-5 screened by the laboratory previously shows effective inhibition against the growth of c. zeae-maydis. the aim of this study was to assess the ability of s3-5 to control gls and to develop a formulation of biocontrol agent with s3-5 to suppress gls. the experiments were conducted on the application of s3-5 spore suspension in the field, as well as screening stabilizers and uv protectants. as a result, the significant control effect was obtained by applying the s3-5 spore suspension twice on maize cultivar zhenghong 311, 2.5% methylcellulose and 0.3% d-sodium isoascorbiate were found as the optimum stabilizer and uv protectant, respectively. this study established foundations for the development of s3-5 biocontrol agent in the future.
keywords: streptomyces lavendulae s3-5;field trial;stabilizers;uv protectants
2018, 44(6): 54-59 收稿日期: 2018-03-02;收到修改稿日期: 2018-04-10
基金项目: 国家国际合作项目（2015dfr31060）
作者简介: 耿琦(1993-),男,陕西镇安县人,硕士研究生,专业方向为应用微生物。
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